Analysis of ?-synuclein levels related to LRRK2 kinase activity: from substantia nigra to urine of patients with Parkinson’s disease

Research on Parkinson’s disease (PD) has been focused the development of PD diagnostic tools as much therapeutics. Several genetic culprits PD, including DJ-1, Leucine-rich repeat kinase 2 (LRRK2), and ?-synuclein (?-syn), have investigated markers in human biofluids. Unfortunately, approaches to develop are impractical, there is a considerable demand for an appropriate marker PD. The measurement ?-syn biofluids recently made more accurate by examining monomers aggregates separately using enzyme-linked immunosorbent assay (ELISA). Previously, we reported two types sandwich ELISA total MJFR-14-6-4-2 antibody-specific fibrillar oligomers. pathogenic LRRK2 G2019S mutation related increased secretion extracellular space. We tested our established differentiated SH-SH5Y cells transfected with G2019S. secretory levels oligomeric divided were significantly G2019S-expressing cells. Additionally, substantia nigra lysates or concentrated urine from patients non-PD subjects analyzed. observed ambiguous changes their ratio between non-PD. Despite insignificant increase relative duration progression after diagnosis corresponded urine. These results might provide greater understanding next stage ELISAs.